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primescript ii reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa primescript ii reverse transcriptase
    Primescript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+ii+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/pm42837891-49-12-16
    Average 96 stars, based on 736 article reviews
    primescript ii reverse transcriptase - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: A Ca 2 ⁺-responsive GH37 neutral trehalase from Lentinula edodes: Cloning, heterologous expression and biochemical characterization.
    Article Snippet: Trehalases (EC 3.2.1.28) hydrolyze trehalose into two glucose molecules and play important roles in fungal trehalose metabolism.. Although GH37 neutral trehalases have been characterized in yeasts and filamentous fungi, their biochemical properties in basidiomycetes remain poorly understood.. In this study, we cloned LeNTH1, a putative GH37 trehalase gene from Lentinula edodes, expressed it in Pichia pastoris, and characterized the purified recombinant enzyme.

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3- step instrument (Roche LightCycler 480).

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Forty-eight hours after transfection, EGFP expression and mCherry fluorescence were analyzed by BD FACS Aria III, BD LSRFortessa X-20 or Beckman CytoFLEX S. Flow cytometry results were analyzed with FlowJo v.10.5.3.

    Reverse Transcription:

    Article Title: A Ca 2 ⁺-responsive GH37 neutral trehalase from Lentinula edodes: Cloning, heterologous expression and biochemical characterization.
    Article Snippet: Trehalases (EC 3.2.1.28) hydrolyze trehalose into two glucose molecules and play important roles in fungal trehalose metabolism.. Although GH37 neutral trehalases have been characterized in yeasts and filamentous fungi, their biochemical properties in basidiomycetes remain poorly understood.. In this study, we cloned LeNTH1, a putative GH37 trehalase gene from Lentinula edodes, expressed it in Pichia pastoris, and characterized the purified recombinant enzyme.

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3- step instrument (Roche LightCycler 480).

    Article Title: Maternal TDP43 orchestrates nuclear speckle assembly and zygotic splicing activation during oocyte-to-embryo transition in mice
    Article Snippet: .. Total RNA was reverse transcribed using PrimeScript II reverse transcriptase (Takara, 6210A) and random primers (Takara, 3801). .. The PCR products were diluted two- to three-fold and subsequently used for semi-quantitative RT-PCR with Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies).

    Article Title: Circ-ZBTB38 as an oncogenic circular RNA: Mechanisms in disrupting RalGAP complexes and its clinical value in melanoma.
    Article Snippet: Objective: Circular RNAs (circRNAs) hold significant potential both in the regulation of tumor progression and in clinical applications for tumors.. This study aims to investigate the functional mechanism of circ-ZBTB38 in melanoma progression as well as its potential clinical application value.. Methods: Differentially expressed circRNAs in melanoma were screened from the GSE138412 dataset and validated by circBase, qPCR, and Sanger sequencing.

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Forty-eight hours after transfection, EGFP expression and mCherry fluorescence were analyzed by BD FACS Aria III, BD LSRFortessa X-20 or Beckman CytoFLEX S. Flow cytometry results were analyzed with FlowJo v.10.5.3.

    Article Title: Targeting notch signaling to restore neural development and behavior in mouse models of ASD
    Article Snippet: Total RNA was isolated using RNAiso Plus (Takara, Kyoto, Japan), followed by DNase treatment with Turbo DNase (Ambion, Austin, TX, USA) to remove contaminating DNA. .. Two micrograms of total RNA were reverse transcribed using random hexamers and PrimeScript II Reverse Transcriptase (Takara). .. Quantitative PCR (qPCR) was performed on a StepOnePlus qPCR system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Master Mix (Applied Biosystems) and the comparative CT method.

    Article Title: The landscape and regulatory potential of eccDNAs in mammalian preimplantation embryos.
    Article Snippet: .. Reverse transcription was performed using PrimeScript II Reverse Transcriptase (Takara) according to the manufacturer’s instructions. .. Quantitative PCR analysis was carried out with Power SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Real-Time PCR system.

    Article Title: Targeting notch signaling to restore neural development and behavior in mouse models of ASD.
    Article Snippet: Total RNA was isolated using RNAiso Plus (Takara, Kyoto, Japan), followed by DNase treatment with Turbo DNase (Ambion, Austin, TX, USA) to remove contaminating DNA. .. Two micrograms of total RNA were reverse transcribed using random hexamers and PrimeScript II Reverse Transcriptase (Takara). .. Nature Communications | (2026) 17:2587 12 Quantitative PCR (qPCR) was performed on a StepOnePlus qPCR system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Master Mix (Applied Biosystems) and the comparative CT method.

    Agarose Gel Electrophoresis:

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3- step instrument (Roche LightCycler 480).

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Forty-eight hours after transfection, EGFP expression and mCherry fluorescence were analyzed by BD FACS Aria III, BD LSRFortessa X-20 or Beckman CytoFLEX S. Flow cytometry results were analyzed with FlowJo v.10.5.3.

    Spectrophotometry:

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3- step instrument (Roche LightCycler 480).

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Forty-eight hours after transfection, EGFP expression and mCherry fluorescence were analyzed by BD FACS Aria III, BD LSRFortessa X-20 or Beckman CytoFLEX S. Flow cytometry results were analyzed with FlowJo v.10.5.3.



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